EWSR1 and cancer: Using cancer cell line RNA-seq data, we first identified 516 genes that were commonly downregulated (>1 FPKM expression and ≥2-fold down) by EWSR1::FLI1 knockdown in three well-characterized EwS cell lines, SK-N-MC, A673, and CHLA-10 (Gollavilli et al., 2018; Riggi et al., 2014).