Furthermore, analysis for the Wnt5a protein by both ELISA assay and flow cytometry–based intracellular staining showed a significant induction of BM stromal Wnt5a protein in CD45–LepR+ cells from WT mice, but not those from FA mice, in response to irradiation (Figure 4B and Supplemental Figure 4C). This evidence concerns the gene PTPRC and Friedreich ataxia.