We additionally included and reclassified 58 spatially separated biopsies from our previous study22 for further characterization of the intratumor heterogeneity in these tumors, which included one IDH-mutant astrocytoma, CNS WHO grade 4, 14 glioblastomas IDH-wildtype, CNS WHO grade 4 and three meningiomas, CNS WHO grade 1. Here, IDH1 is linked to astrocytoma (excluding glioblastoma).