To study the dynamics of cytoskeletal structure during glioblastoma subtype transition, the cell line model used included cell lines U-2982MG, U-2982MG/YFP, U-2982MG/SOX2, U-2987MG, U-2987MG/YFP, and U-2987MG/SFRP2, which thus represented two cell types with mesenchymal subtype gene expression signature (i.e. U-2982MG and U-2987MG/SFRP2), two cell types with proneural subtype gene expression signature (i.e., U-2982MG/SOX2, and U-2987MG), and two transfection control cell lines (i.e., U-2982MG/YFP and U-2987MG/YFP) (Fig. 3f)29. This evidence concerns the gene SFRP2 and glioblastoma.