To accomplish this dual purpose we first utilized LLC-conditioned media (LCM) as a strategy to recapitulate the cachectic effects of cancer in vitro (Zhang et al. 2011; Puppa et al. 2014; Brown et al. 2018), followed by LLC-induced cachexia in vivo with both wildtype (WT) and muscle-specific PGC-1α overexpressing (MCK-PGC-1α) mice. This evidence concerns the gene PPARGC1A and cancer.