INS and obesity due to melanocortin 4 receptor deficiency: When compared with age- and sex-matched mice fed on RC (n = 7), mice fed on a NASH diet (n = 7) developed obesity (body weight, 49 ± 2.9 versus 37 ± 1.6 g in RC control, P < 0.001), with a concomitant induction of fasting hyperglycemia (blood glucose, 135 ± 6 versus 105 ± 7 mg/dL in RC control, P < 0.001) and fasting hyperinsulinemia (plasma insulin, 2.09 ± 0.75 versus 0.17 ± 0.04 ng/mL in RC control, P < 0.001), suggestive of insulin resistance, as reflected by HOMA-IR (17.3 ± 6.6 versus 0.7 ± 0.15 in RC control, P < 0.001) (Supplemental Table 1).