FOXO1 and Insulin resistance: To characterize the role of FoxO1 in macrophage activation and determine the macrophage FoxO1 contribution to hepatic inflammation and NASH, we generated myeloid cell conditional FoxO1-knockout (FoxO1-KO) mice, followed by determining the effect of FoxO1 loss of function on macrophage activation and polarization in response to overnutrition and insulin resistance.