ACTA2 and metabolic dysfunction-associated steatohepatitis: We demonstrated that hepatic expression of TGF-β, a key fibrogenic factor along with its downstream targets Acta2, Col1a1, Col3a1, Timp1, Mmp2, Mmp12, and Mmp13 was significantly reduced in MøFoxO1-KO versus WT littermates on a NASH diet (Figure 6F).