INS and Hyperinsulinemia: In contrast, HFD-induced hyperinsulinemia was significantly reduced (fasting plasma insulin, 0.5 ± 0.07 ng/mL in MøFoxO1-KO versus 1.8 ± 0.35 ng/mL in HFD-fed WT littermates, P < 0.01), with a concomitant restoration of glucose-stimulated insulin secretion in MøFoxO1-KO mice (Figure 2C).