These results were corroborated by the determination of hepatic mRNA profiles, demonstrating that hepatic expression of FoxO1 and inflammatory cytokines IL-1β, TNF-α, IL-6, Ccl2, and Ccl3, along with Ccr2, were significantly upregulated in NASH diet versus RC mice (Supplemental Figure 4). This evidence concerns the gene FOXO1 and metabolic dysfunction-associated steatohepatitis.