To verify the expression of EMT-related prognostic signatures, including BDNF, COPA, GADD45B, GPX7, ITGB5, LOX, MATN3, MCM7, MMP1, and SPP1, in HCC cell lines, we conducted a qRT-PCR analysis to determine mRNA expression. The gene discussed is COPA; the disease is hepatocellular carcinoma.