In the DSS group, our data showed that although TLR4 and IRAK1/4 were significantly elevated, IRAK1/4 expression decreased after inhibiting IRAK1/4 activity, using the IRAK1/4 inhibitor, effectively reducing the expression of NF-κB and its downstream inflammatory molecules in the intestines of colitis mice. This evidence concerns the gene TLR4 and colitis.