In this study, although only the basic pharmacological inhibition effects of the IRAK1/4 inhibitor on IRAK1/4 activity in mice with colitis were preliminarily analyzed, the relationship between the inhibition of IRAK1/4 activity, the TLR4-NF-κB pathway activity, and improvement of intestinal barrier function was discussed. The gene discussed is TLR4; the disease is colitis.