The enrichment for mutations in mprF, rpoB, stp1, sgtB, and in the walKR/yycH operon (11-fold enrichment, p=9 × 10–9, see Figure 5—figure supplement 1 for the operon enrichment analysis) highlights the role of antibiotic pressure in shaping adaptation within the invasive population, since these loci are hotspots of adaptation to key anti-staphylococcal antibiotics that are often used in invasive infections (rifampicin, daptomycin, and vancomycin). The gene discussed is SGTB; the disease is infection.