To determine how MUC1 depletion would impact IAV infection dynamics, we inoculated both MUC1 KO and control HAE cultures with 500 PFU (approximate MOI of 0.01) A/Udorn/307/72 to allow multiple rounds of infection and monitored both viral growth kinetics as well as spread throughout the culture by en face staining for viral antigen. This evidence concerns the gene MUC1 and infection.