After injection of EVs or EVs‐miR‐200b‐3p in MI mice, malondialdehyde and ROS contents were suppressed and SOD activity was strengthened, LVEDD, and LVESD were reduced, LVEF and LVFS were enhanced; the improvement effect of EVs‐miR‐200b‐3p treatment was better than EVs treatment. Here, SOD1 is linked to myocardial infarction.