SNRPN and Prader-Willi syndrome: It was reported that DNA extracted from dried blood spot (DBS) could be analyzed by the methylation-sensitive high-resolution melting (MS-HRM) methodology, which used a unique pair of primers to amplify the promoter-exon 1 region of the SNURF-SNRPN locus and reveal its methylation status [57], providing an accurate approach for genetic screening of PWS in newborns [71].