Moreover, we used western blotting to detect the Cit-H3 protein level and found that Cit-H3 expression was highest in ARDS neutrophils with GM-CSF and LPS, and the situation was reversed by AT7519 stimulation (Fig. 7C and D; GM-CSF + PMA group vs. GM-CSF + AT7519 + PMA group, p = 0.0046, LPS + PMA group vs. LPS + AT7519 + PMA group, p = 0.0012). Here, CSF2 is linked to acute respiratory distress syndrome.