We found the combination of CGRP and PACAP-38 had the best diagnostic value in pediatric migraine (AUC: Combination (0.926) vs CGRP (0.869) vs PACAP-38 (0.867)) and had the highest specificity (specificity: Combination (90.91%) vs CGRP (76.62%) vs PACAP-38 (71.43%)). The gene discussed is ADCYAP1; the disease is migraine disorder.