We observed that mRNA levels of podocytes markers, such as nephrin (Figure 5A) and podocin (Figure 5B), were reduced with the increased mRNA level of muscle marker, desmin (Figure 5C), which suggested that podocytes were reduced in the kidney of DKD rats and restored by high dose of Qidantang Granule. This evidence concerns the gene NPHS2 and diabetic kidney disease.