Moreover, we further tested the impact of most popular AD risk gene (ApoE genotypes) on autophagy pathways through our 3D iPSC‐derived brain organoids or iPSC‐derived assembloid system (mixed culture model; organoid + microglia) using CRISPR‐Cas9‐based sporadic AD modeling iPSC lines (ApoE ɛ3/ɛ3 iPSC, parental line; ApoE ɛ4/ɛ4 iPSC, isogenic line) (Figure 7a, validation I‐ii; related to Figure 7d–7f). Here, APOE is linked to Alzheimer disease.