However, these results are in stark opposition to results generated from aggrecan or brevican labeling of the PNN CSPG core proteins in AD postmortem brain tissue, which showed either no change by immunohistochemistry (Brückner et al., 1999; Morawski et al., 2010a, 2012) or an increase in abundance by western analysis (Lendvai et al., 2013; Howell et al., 2015). This evidence concerns the gene ACAN and Alzheimer disease.