ANXA5 and infection: However, inhibition of PMN glycolysis with 2-DG prior to infection ablated the ability of LVS to extend neutrophil lifespan and at the same time accelerated constitutive apoptosis of uninfected PMNs as indicated by detection of externalized PS by Annexin V-FITC staining at 10 hr (p < 0.0001) (Figure 5A).