PRF1 and neoplasm: Indeed, if the expression of GSDME in tumor cells was up-regulated accompanied by increasing tumor-infiltrating NK and CD8+ T lymphocytes, these killer cells could release perforin to form pores on their own cell membrane, permitting granzyme B to burst into the cytoplasm of target tumor cells, thereby cleaving GSDME after D270 to induce pyroptosis in a similar manner to caspase-3 (99).