Here, the nano vaccine is composed of a mixture of mRNA species encoding the melanoma tumor antigens New York esophageal squamous cell carcinoma (NY-ESO-)1, melanoma-associated antigen (MAGE-)3, tyrosinase and putative tyrosine-protein phosphatase (TPTE), and cationic liposomes, which upon intravenous injection effectively transfected APC such as DC and macrophages, thereby inducing strong tumor antigen-specific CD4+ and CD8+ T cell immunity (109). This evidence concerns the gene CD4 and neoplasm.