TGFB1 and systemic sclerosis: 1,25(OH)2D3 also induced a significant reduction of TFGβ synthesis and mRNA expression in SSc patients, but only at the highest concentration (10-7M: 616.8±202.3 vs 784.11±239.6 pg/ml, Figure 3; relative mRNA levels from 1.4-fold in untreated cell to 0.80, p<0.01 compared to control); similarly, in PBMCs from healthy subjects, treatment with the highest concentration of 1,25(OH)2D3 significantly reduced TGFβ synthesis and mRNA expression (10-7M: 578.91±101.4 vs 663.75±84.9 pg/ml, p<0.05, Figure 3; relative mRNA levels 0,80-fold compared to control, p<0.05).