ROC curve analysis showed that the AUC of LH peak in diagnosing girls with precocious puberty was 0.973, which was higher than 0.895, 0.875, and 0.912 of LH base value, FSH base value, and FSH peak value, respectively, as shown in Table 3 and Figure 1. The gene discussed is PLOD1; the disease is precocious puberty.