After the ATF-2 gene was knocked out, the expression of pathway genes decreased, and the proliferation and invasion ability of NSCLC cells decreased; after gene knockout and agonist addition, the expressions of Wnt5a and other proteins increased, and the proliferation and invasion ability of NSCLC cells also increased, which was similar to the blank control group. The gene discussed is ATF2; the disease is non-small cell lung carcinoma.