Next, we investigated by immunoblot and immunofluorescence the expression and subcellular localization of PDK2 and PDK3 (tagged with a Flag epitope at the C-terminus) ectopically expressed in COS-7 (as a suitable cell model for ectopic protein expression) and LNCaP PCa cells (Figures 4A–D). The gene discussed is PDK2; the disease is posterior cortical atrophy.