First, using the CIBERSORT method and the Wilcoxon rank sum test, we determined the proportion of each immune cell type in the COAD patient cohort (Figure 6A), and some relationships between immune cells were strong, such as the relationships between resting memory CD4+ T cells and M0 macrophages (r=-0.43), CD8+ T cells and resting memory CD4+ T cells (r=-0.26), and CD8+ T cells and M0 macrophages (r=-0.42) (Figure 6B). This evidence concerns the gene CD4 and colon adenocarcinoma.