For example, EVs-miR-1247-3p secreted by HCC cells directly transferred to lung pre-metastasisniche fibroblasts, decreased the expression of β-1,4-galactosyltransferases III (B4GALT3, a protein mediating glycosylation), thereby converting them into CAFs, and then activated the β1-integrin-NF-κB signaling pathway to promote EMT, thereby promoting the metastasis of hepatocellular carcinoma to the lung, and IL-6 and IL-8 secreted by CAFs to promote the development of HCC (Figure 3.1) (143). This evidence concerns the gene CXCL8 and hepatocellular carcinoma.