To confirm that this interaction also occurred in breast cancer cells, we firstly investigated the location of TRAF4 and Eg5 in MDA-MB-231, SK-BR-3, MCF-7 and MDA-MB-453 breast cancer cells, and found both TRAF4 and Eg5 have cytoplasmic localization in these cells (Figure 2A). Here, TRAF4 is linked to breast carcinoma.