The results showed that the growth of tumors over-expressing TRAF4 was significantly accelerated, and when Eg5 was depleted at the same time, the promotion effect of TRAF4 on tumor growth was inhibited (Figure 6E), verifying that TRAF4 promotes the proliferation of breast cancer cells by up-regulating the protein level of Eg5. The gene discussed is TRAF4; the disease is breast carcinoma.