Our study identified a hypoxia-associated lncRNA USP2-AS1.And we observed USP2-AS1 was significantly up-regulated in HCC compared with normal liver tissue, patients with low expression of USP2-AS1 had better prognosis while patients with high expression of USP2-AS1 had shorter OS and DFS time. This evidence concerns the gene USP2 and hepatocellular carcinoma.