Morever, in consistant with other studies in 2D culture, we observed that the ptau level was also significantly reduced in in NE4 + AE4 chCOs after inhibiting cholesterol synthesis by atorvastatin and simvastatin, suggesting targeting cholesterol metabolism is a valuable strategy in developing therapeutic agents for AD patients carrying APOE4.28 Wang et al.27 recently report that cholesterol is synthesized in astrocytes and transferred to neurons in apoE lipoprotein particles. This evidence concerns the gene APOE and Alzheimer disease.