The finding that increased levels of THBAs are associated with a protective effect against cholestatic liver disease is in line with what was seen in WT mice subjected to common bile duct ligation after treatment with constitutive androstane receptor and pregnane X receptor agonists regulating the BA hydroxylation enzymes Cyp2b10 and Cyp3a11. This evidence concerns the gene NR1I2 and Cholestatic liver disease.