NBL is a heterogeneous tumor and different clinical parameters contribute to the survival of NBL [56, 58], as previously mentioned, a significant difference across immune subtypes within the high risk NBL without MYCN amplification was observed (Fig. 3C, D, Supplementary Table 4), we performed multivariate analysis to correct for the contribution of other clinical parameters in the survival of NBL, a significant difference in survival was found between S2 (p = 0.0319) and S6 (p = 0.0452) compared to S4. The gene discussed is MYCN; the disease is neoplasm.