To further confirm the role of Acp5 in pyramidal neurons of PrL in the comorbidity of neuropathic pain and depression in rats, we overexpressed the Acp5 by bilateral injection of AAV–DIO–Acp5–EGFP into the PrL together with AAV–CaMKIIa–Cre (Fig. 5A, B). The gene discussed is ACP5; the disease is major depressive disorder.