In this study, the potentially interactive combinations gga-miR-7460-3p/PIPK2 and gga-miR-145-5p/MALT1 at both time points, gga-miR-214/STAT3 at 10 DPI, and gga-miR-12,265-5p/IRF4 at 15 DPI may play the key roles in regulation of the Th cells responses in chicken cecum tissue infected with H. meleagridis. This showed that the host initiated different signaling pathways by the miRNAs and their targets to regulate Th cell differentiation to cope with the development of infection. Here, MALT1 is linked to infection.