Methylation of Wnt family member 10A (WNT10A)11, neuropeptide Y (NPY)11, nestrogen receptor 1 (ESR1)12, estrogen receptor 2 (ESR2)13, protocadherin 10 (PCDH10)14, cartilage oligomeric matrix protein (COMP)15, hyaluronan synthase 2 (HAS2)16, pituitary homeobox 1 (PITX1)17, histone H3 lysine 9 (H3K9)18, and necdin (NDN)19 genes were reported to be relevant to AIS. The gene discussed is ESR2; the disease is androgen insensitivity syndrome.