However, both cardiac gene expression analyses and immunohistochemistry revealed that at 3 weeks post-TAC there was only a slight, non-significant increase in cardiac Vegfc and Vegfd levels (see Supplementary material online, Figure S2a), and in lymphatic density (Figure 2b), in C57Bl/6 males, despite their extensive cardiac hypertrophy. Here, VEGFD is linked to cardiac hypertrophy.