In addition, IHC analysis also showed that NCAPD3 knockout led to remarkably weaker staining of c-Myc, E2F1 and GLUT1 (Fig. 7E), as well as a significant decrease of the proliferation rates of colorectal tumors in NCAPD3± mice, as evidenced by lower proportions of Ki-67 nuclear staining (Fig. 7E). This evidence concerns the gene MKI67 and colorectal neoplasm.