Moreover, immunoprecipitation assays showed that E2F1 could be co-precipitated by anti-NCAPD3 antibody in HCT116 cells or SW480 cells with NCAPD3 knockdown (Fig. 4C, D), suggesting that NCAPD3 and E2F1 interacted in CRC cells. Here, NCAPD3 is linked to colorectal carcinoma.