AKT1 and Hyperglycemia: Our results confirmed that a high level of insulin and hyperglycemia decreases AMPK activity through phosphorylation of AMPK subunits by the PI3K/AKT pathway (Juszczak et al. 2020), where the protein levels of AMPK and PI3K/AKT were downregulated after 30 min of renal ischemia and 48 h reperfusion.