To screen the downstream genes of VDAC1 involved in the tumorigenesis and progression of HCC, we used Affymetrix GeneChip analysis for gene expression profiling to compare three VDAC1-knockout Huh7 cell lines (E5176-1, E5176-2, E5176-3) and three negative control Huh7 cell lines (E5175-1, E5175-2, E5175-3). Here, VDAC1 is linked to hepatocellular carcinoma.