MLKL and acute kidney injury: In the pig model of I/R-induced AKI, we observed apoptotic renal tubular cells, as shown by TUNEL positive staining (Figures 2A,B) and increased expression of cleaved caspase-3, a major apoptotic marker (Figures 2E,F), and necrotic tubular cells, as evidenced by increased phosphorylation levels of MLKL and RIPK3, two markers of necroptosis, by both immunofluorescent staining (Figures 2C,D) and immunoblotting (Figures 2E,G–J).