TIMER2.0 revealed that the expression of TIMP1 was remarkably associated with the infiltration of CD4+ T cell (Rho = 0.335, P = 6.28e − 05), B cell (Rho = 0.408, P = 7.63e − 07), T cell regulatory (Tregs) (Rho = 0.514, P = 1.29e − 10), neutrophils (Rho = 0.312, P = 2.10e − 04), cancer-associated fibroblast (Rho = 0.653, P = 5.46e−18), macrophage M1 (Rho = 0.492, P = 1.04e − 09), and myeloid dendritic cell (Rho = 0.338, P = 5.40e − 05) in GBM. The gene discussed is CD4; the disease is glioblastoma.