The results show that in osteosarcoma tissues inoculated with cells stably transfected with circSRSF4 siRNA, the expression of circSRSF4 was significantly lower than that of the control group (Figure 6C), the expression of miR-224 was significantly higher than that of the control group (Figure 6D), and the expression of Rac1 was significantly lower than that of the control group (Figure 6E,F). The gene discussed is RAC1; the disease is osteosarcoma.