Subsequently, in the present work, to address the problem of the dysregulation of TERRA, TERC and TERT mRNA in HCC from a biological point of view, we characterized two HCC cell lines for the expression of these transcripts and, for the first time, we report data on their expression both at the cellular and extracellular levels, indicating that the two HCC cell lines may represent the heterogeneity of HCC. This evidence concerns the gene TERT and hepatocellular carcinoma.