ESR2 and obesity due to melanocortin 4 receptor deficiency: We selected miRNAs for this analysis that met three primary criteria: (i) a predicted in silico interaction with ESR1 or ESR2 mRNA, (ii) a suggested role in the regulation of ESR1 or ESR2 expression in vivo, and (iii) an altered expression in adipose tissue in the course of obesity or weight loss.