ESR2 and obesity due to melanocortin 4 receptor deficiency: However, the methylation status of the analyzed regions did not correlate with the ESR2 mRNA levels, Moreover, no significant differences in the methylation statuses were found between the DNA samples obtained from the VAT and SAT of (i) obese and normal-weight study participants and (ii) metabolically healthy obese subjects and those with the metabolic complications of obesity (Supplementary Table S2).