We used next-generation sequencing of total RNA isolated from the interventricular septum of sham- and TAC-operated AAV9-Luc (sham: n = 3; TAC: n = 4) and AAV9-Tfeb (sham: n = 3; TAC: n = 3) mice to quantitate TFEB-dependent gene expression. The gene discussed is TFEB; the disease is persistent truncus arteriosus.