In this study we describe the technical approach during the implementation of two panels of validated antibodies to evaluate the proliferation rate of tumor cells based on Ki67 signal, tumor infiltrating immune cells by CD8 signal, and by CD68 detection for macrophages, and the tissue localization of the immune checkpoint PD-L1, applying tissue segmentation and cell phenotyping to interrogate the cytokeratin positive and negative compartments in retrospective cases of rare cancer in Chilean patients. This evidence concerns the gene CD68 and cancer.