We further confirmed the effect of penfluridol on glycolysis by checking several key glycolytic enzymes and found that HK, PFKP, and PKM2 were upregulated by penfluridol treatment in A549 cells in a time-dependent manner, but PDH was downregulated (Figure 3A), suggesting that penfluridol actually triggered glycolysis in NSCLC cells. The gene discussed is PKM; the disease is non-small cell lung carcinoma.