In penfluridol-treated A549 cells, we found that SIRT1 and p53 were significantly downregulated by penfluridol treatment, but phosphorylated (p)-AMPK was concentration-dependently upregulated by penfluridol (Figure 2F), suggesting that penfluridol may regulate PGC-1α in NSCLC cells via transcriptional regulation and PTMs. This evidence concerns the gene PPARGC1A and non-small cell lung carcinoma.