Monokine induced by gamma interferon (MIG), interleukin 22 (IL-22), and tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) showed an AUC value above 0.80, and A proliferation-inducing ligand (APRIL), VEGF, IL-3, the cytokine TNF-related weak inducer of apoptosis (TWEAK), stem cell factor (SCF), and IL-21 even above 0.9, in discriminating patients developing de novo HCC after DAA treatment. The gene discussed is IL22; the disease is hepatocellular carcinoma.