Western blot analysis was performed to detect three EV markers (i.e., Alix, Tsg101 and Hsp70), a negative control marker calnexin (an ER marker), and MYCN encoding n-Myc protein in the cell lysate, MV and Exo samples of MYCN-amp (SK-N-BE2) and MYCN-NA (SH-SY5Y) NB cell lines (Figure 1a). Here, MYCN is linked to neuroblastoma.