To assess the generalizability of MYCN-MV detection as the liquid biopsy of MYCN-amp NB, six cell lines including three MYCN-amp (SK-N-BE2, SK-N-DZ, IMR-32) and three MYCN-NA (SH-SY5Y, SK-N-AS, SK-N-SH) NB cells were propagated and maintained for further experiments. Here, MYCN is linked to neuroblastoma.