LDLR and familial hypercholesterolemia: Based on our previous studies showing the emergence of foamy monocytes in mice with hypercholesterolemia and their contributions to atherogenesis, [8,12] we assessed lipid accumulation, indicated by the FACS side scatter (SSC, representing cell granularity and accumulation of lipid droplets) with Nile red staining, and the phenotypes of circulating monocytes in Ldlr−/− mice receiving P407 and the saline control.