These currents were completely abolished in the HCT116 TRPM4 knock-out cells, M4KO 1–5 (Figure 1A,B), which confirmed our previous results that identified TRPM4 as the main source of ICAN (Ca2+-activated nonselective current) in the CRC cell line HCT116 [32]. This evidence concerns the gene TRPM4 and colorectal carcinoma.